"Nanoparticle in Flow Cytometry Workshop" was held

'Nanoparticle in Flow Cytometry Workshop ' was held by Üsküdar University, Faculty of Engineering and Natural Sciences, Department of Molecular Biology and Genetics and TRGENMER. In the workshop, in which it was emphasized that it is very important to characterize exosomes after isolating them, and it was underlined by experts that exosomes and lipid nanoparticles are not newly discovered methods. In addition, it was emphasized that the vesicles of lipidomic profiles are also very important. It was pointed out that half of the studies conducted so far are on cancer.

The event was attended by the Director of TREGENMER, Üsküdar University Faculty of Engineering and Natural Sciences, Department of Molecular Biology and Genetics, Asst. Prof. Cihan Taştan, Üsküdar University Department of Molecular Biology and Genetics faculty member Prof. Sevim Işık, Beckman Coulter Company Senior Development Specialist Gülhis Akar and Gaziantep Islamic Science and Technology University Dr. Mustafa Öztatlıcı.

Students of the Department of Pharmacy and the students of the Department of Molecular Biology and Genetics came together at the event held at Üsküdar University Central Campus Nermin Tarhan Conference Hall.

Asst. Prof. Cihan Taştan: "It is very important to be able to characterize exosomes after isolating them"

Üsküdar University, Faculty of Engineering and Natural Sciences, Department of Molecular Biology and Genetics, Asst. Prof. Cihan Taştan shared the presentation titled 'Development and Application Areas of Transgenetic Exosome Products' with the participants. Pointing out that it is not possible to collect exosomes only from the cell, Taştan said that "We mostly work by genetically modifying these cells. If you can make a genetic modification to a variety of cells, then when you focus the gene that you are going to produce on the exosome, and you can change not only the surface of the exosome, but also the cargo that it carries. To achieve this, you must perform your DNA design. You can transfer it into the cell, sometimes with a permanent perm and sometimes with a normal transfection method. Of course, it is not possible to collect exosomes only from the cell. Here, it is very important to be able to characterize exosomes after isolating them, rather than their production because it is important to perform characterization processes such as whether the inside of each exosome you collect is full, and whether the relationships in the cells to which the carrier system will be connected exist correctly."

"Exosomes and lipid nanoparticles are not newly discovered methods"

Expressing that the number of articles on exosomes has increased rapidly in recent years, Asst. Prof. Cihan Taştan stated that "Especially after the covid pandemic, we have started to see exosomes more in recent years, with studies such as the ability to package genetic materials such as mRNA under its value, to create an immune reaction, and to increase its stability. Of course, exosomes and lipid nanoparticles are not newly discovered methods. The history of this goes back to 1965. In these years, it was shown whether exosomes that were first discovered or isolated later carried antigens and that these exosomes were not actually used only as a transfer agent. Of course, with the discovery of these and the subsequent emergence of the idea that they can be used to kill cancer, we can now isolate exosomes from natural killer cells or other immune system cells and use them in treatments such as cancer."

Prof. Sevim Işık: "Exosomes have the characteristic feature of the cell"

Department of Molecular Biology and Genetics faculty member Prof. Sevim Işık informed the participants with her presentation titled 'Exosome Applications'. Stating that the biggest problem in brain diseases is to send cells to the brain, Prof. Sevim Işık said that "When you look at exosomes, we just call them natural drug delivery systems. Before that, liposomes were already able to do this job. As a transport system, we could direct them in a targeted way; however, the advantage of exosomes is that you have the characteristic of the cell in your hand. If it is a stem cell or, for example, an immune system cell, it adds its telepathic quality. You enrich it. Naturally, it is already a goal-oriented transport system... For example, we have the biggest problem in brain diseases when we send the cells to the brain. They cannot cross the brain barrier, or they pass very limitedly. Due to their small structure, you can send them in any direction you want, like navigable weapons. Therefore, it is a very important area."

Dr. Mustafa Öztatlıcı: "The vesicles of lipidomic profiles are also very important"

Dr. Mustafa Öztatlıcı from Gaziantep Islamic Science and Technology University made a presentation titled 'Extracellular Vesicles: Nano-Messengers in Intercellular Communication'. Emphasizing that vesicles are not only obtained from mammalian cells, but Dr. Mustafa also Öztatlıcı said that "Vesicles are not only derived from mammalian cells. It is also obtained from mushrooms, alps, and plants. Therefore, the communities working in this field name themselves as Extracellular Vesicles. After the study conducted in 2007 determined that they carry micro-RNA and mRNA, there is an increasing interest in this field. We can obtain these vesicles in many fluids in our body. The vesicles of lipidomic profiles are also very important. After the publication in 2007, the interest in this field has increased."

Gülhis Akar: "Half of the studies conducted so far are on cancer"

Senior Development Specialist at Beckman Coulter Company Gülhis Akar shared her presentation titled 'CytoFLEX and Extracellular Vesicle Studies' with the participants. Stating that IV is quite important, Akar said that "These are interesting topics for us and for you because all cells are produced by systems. Cargo elements that carry interfaces, proteins, mRNAs, antigens for us to study physiology and pathology... Due to their size and scope, they can be used in all kinds of research. These are rare structures that cross the blood-brain barrier. In general, all the research, which corresponds to half of the studies conducted so far, is more focused on cancer, that is, it is mostly studied on cancer. Currently, 61 clinical trials are ongoing involving IVs. As a company, we are on the side of how IVs look and how they are used. The issue of IV is very important and trendy as I call it.”

After the group photo shoot, the event ended.

Üsküdar News Agency (ÜNA)